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[KO]TAF15 Rabbit mAb

Western blot analysis of TAF15 in Caco-2(WT) and TAF15-Knockout Caco-2(KO) lysates using TAF15 antibody.
Western blot analysis of TAF15 in K562, rat Brain, C6, 3T3 lysates using TAF15 antibody.
Immunocytochemistry analysis of TAF15(green) in Hela using TAF15 antibody,and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using TAF15 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC
Primary Accession	Q92804
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Purification	Affinity Purified
Calculated MW	61830 Da

Additional Information
Gene ID	8148
Other Names	TAF15
Dilution	WB~~1:1000-1:5000 IHC-P~~1:50~200 FC~~1:50-1:100
Format	Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TAF15
Synonyms	RBP56, TAF2N
Function	RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed:21256132). Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Cellular Location	Nucleus. Cytoplasm. Note=Shuttles from the nucleus to the cytoplasm
Tissue Location	Ubiquitous. Observed in all fetal and adult tissues

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).

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¥ 1,250.00

Cat# AP76146

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[KO]TAF15 Rabbit mAb

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